Part:BBa_K2018014:Experience
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Applications of BBa_K2018014
We cloned the part into a purification plasmid (pTXB1) provided from NEB, we then performed a impact purification of 500 ml ON-cuture and ended with 1.5 ml purified protein with a concentration of 52,5 ug/ml.
We then performed a MIC assay to test for antimicrobial effect. The MIC assay where performed on three resistant S. aureus and one P. aeruginosa we got the following results:
| Strain | ug/ml |
| S. aureus:CC398 | 13.1 |
| S. aureus:USA300 | 13.1 |
| S. aureus:hVISA | 6.6 |
| P. aeruginosa:PAO1 | 13.1 |
The idea is that you can use the bacteriocin as a antimicrobial agent how ever it pleases you. We looked into the possibility of coating both the bacteriocin in a compound to make it not digested, or coating stuff in bacteriocins so they may be antimicrobial.
User Reviews
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